Description:
History of chromatography and the separation methods.
Introduction, theory, Aim, essential terminology
Paper ,thin layer and column chromatography
HPLC Instrumentation overview and practical theory
Different types of detectors
Columns
Qualitative Analysis
Quantitative analysis
GC Instrumentation overview and practical GC theory:
Packed and capillary techniques
Chromatography classification, GC Familiarization and GC   
Techniques GLC & GSC
Factors affecting peak order, the optimum condition for good     
resolution of GC and mechanism of separation.
Overview of Sampling Techniques of gas chromatography

(Gases and Liquids), sample containers.
Different types of detectors.
Qualitative AnalysRetention data
Retention indices.
Relative retention
Quantitative analysis.
Area normalization and Response factor.
Relative response factor.
Comparison with standard mixture.
Absolute calibration and Internal standard method.